Carolina Tiger Rescue Holiday Update

For those of you who don't know, Carolina Tiger Rescue is my 'home territory,' the place that first introduced me to and helped me learn about so many exotic cat species. The people and the animals at this sanctuary are so amazing! I love each and every creature- two legged and four. The sanctuary is home to tigers, ocelots, caracals, servals, one bobcat, kinkajous, and binturongs. For those close by, now is a great time to consider becoming a member, volunteer, or adoptive parent at Carolina Tiger! The sanctuary will be receiving six new cats in the next few weeks: three lions and three tigers from a closing facility in Texas. With these new additions, help is always needed to continue ensuring the high quality of life maintained at Carolina Tiger Rescue. Also, with the holidays approaching, the priority item on their wish list are Motorola radios for animal care and safety coordination. Having dropped one of these radios into a five gallon bucket of water (oops), I know how heavily they are used and how essential they are to getting things done on the 55 acre facility. The sanctuary always welcomes new volunteers, so please visit their website to learn about new volunteer orientations. Adoptive parents donate a fixed rate in honor of the of the resident animals, thereby becoming the animal's "adoptive parent." Adoptive parents enjoy visits, enrichment creation, and playing active roles in the care of those animals. And finally, if you are looking for a way to give during the holidays, consider a charitable donation for the animals at Carolina Tiger Rescue. This might be cliche, but they really are some cool cats!

And if you aren't nearby or cannot afford the time to visit Carolina Tiger Rescue, visit the website to learn about how shopping Pampered Chef with give a portion of the proceeds to Carolina Tiger Rescue (good through December 6).

http://carolinatigerrescue.org/


Be sure to become a fan on facebook!
http://www.facebook.com/index.php?lh=441fd662b1e57dec8cde6d775d66cf2f&#!/CarolinaTigerRescue


Julio, one of my favorite ocelots, with his holiday pumpkin (October 2009)

Rat, But Not the Rodent

The project I'm working on has been a long time coming; clouded leopards have very unique reproductive problems, unfortunately which cannot be fixed with the snap of a finger (were this the case, I'm pretty sure I'd be famous right now). I'm working on the female side of things, but there is also a male aggression study going on that a new intern just arrived for. I'm working for a pretty cool lady named JoGayle Howard. JoGayle is quite literally the clouded leopard queen: she's headed up most of the studies and the advances in clouded reproduction. She's a theriogenologist, or a veterinarian who focuses on reproduction, and a PhD at the Smithsonian. She's done everything, from developing successful cryopreservation of sperm, laparoscopic artificial insemination in carnivores, and genome banking. In short, she's pretty awesome. Which by default, the project I'm working under, under the supervision of postdoc Rebecca Hobbs, is also awesome. For more insight into the project, check out this website:

http://cloudedleopard.org/default.aspx?link=research_inzoos

Having been here in Front Royal for a month, I've finally gotten into the swing of things with the project. I've cataloged, dried, and sorted more fecal samples than I care to know, and developed a system for which to extract each sample set. I'm using a boiling extraction process, which is pretty simple: fecal sample are collected from each animal over a pre-determined time period (one sample, or one week's worth, is not enough to quantify hormone changes in reaction to the study protocol); freeze-dried of all water (called lyophilization); and crushed and weighed out into test tubes. Once in the tubes, I add radioactivity that will later determine the extraction efficiency of each sample; that is, the radioactivity provides a way to show how much hormone was pulled from the fecal sample during the extraction process. This is essential because later steps will be measuring hormone levels, such as estrogen and progestogen. The extraction of this whole process takes place with the addition of ethanol and boiling the mixture for twenty minutes (smells great), then centrifuging (think worst merry-go-round ride ever), then centrifuging some more with some more ethanol, and drying down the supernatant two different times. There are more in-between steps, with some methanol and dilution buffer and a sonicator, the machine that cleans jewelry, but these are the basics. After I have my final dilution, called a cocktail (no joke!), I throw all the samples into a beta counter, a machine which measures the radiation emitted by beta-emitting nucleotides via light pulses (the scintillation fluid I add to the cocktail throws a nice prism ). In short, I find out if I did the whole process right and have proper extraction efficiencies for my samples. Usually, I do. I've got to re-run about twenty samples, and so far I've run almost 600. Once I'm done extracting all these samples, I'll hopefully have time left during the internship to run enzyme immunoassay plates (EIA) for the hormones. I'm looking forward to more procedures under the guidance of the lab manager and the dugong project being run by a visiting scientist from Australia. Consequently, I see a lot of this:





I guess this makes me a lab rat!

I've also looked into volunteering with the clouded leopards on site. I've had a good peek at two eight-month old leopards that are visiting, and I'm not sure there is anything more beautiful, or adorable, than these rascals. Although the clouded animal keeper, Jessica, might think otherwise of the very-demanding squawking these two cats can emit. I can hear it all the way down the hall!

Finally, a few of us went hiking in the Shenandoah last weekend. We had so much fun! Martin is a GIS intern who just arrived from Germany, so we took him out to see the last of the changing leaves. Two hikes and 7.5 miles later, we had seen a gorgeous peak and a lovely waterfall. I wondered why I was so exhausted during the first hike (it's not like I hiked every day this past summer), but then I realized that we were on the hike that, in less than a mile, you climb 900 ft in elevation. That's a lot of climbing and hating, in case you were wondering how to measure that. Once we got to the vista, however, all huffing and puffing was lost in the view of the Shenandoah valley in the fall. Scaling huge rocks, we sat atop the valley for almost an hour, taking in the view and having a Lion King moment or two (think Pride Rock).





Hike two was longer, but easier, and took us along a beautiful creek down to a waterfall. Instead of hiking to the cliff overlooking the lengthy fall, we opted to hike down into the midst of it. The slope wasn't very steep, but the view and the sounds were beautiful:



All in all, so far so good. I must get to bed- I've got to get into the lab early tomorrow!

Autumn Conservation Festival

I've been living at and working at the Smithsonian Conservation Biology Institute (SCBI) for four weeks now, and it has been pretty busy! I've really enjoyed getting to know each of the other interns, their interests, and their stories. We come from all over the country and all over the world, from Australia to Canada to Germany, and each individual is passionate for either a particular species, or a particular science. One of the girls is a visiting PhD student from Australia studying dugong endocrinology at a coastal research institute I actually visited while in Australia (dugongs are the Aussie version of manatees)! The intern lab trainer is doing her master's on lion endocrinology (the lionesses at the National Zoo had their cubs this fall!), and another two interns completed their master's in primate and elephant conservation, respectively. The current cheetah intern worked at the Iditarod this summer, and another is working towards vet school. The people here are so interesting! Be it education, endocrinology, veterinary medicine, or conservation ecology, this facility is full to the brim with bright minds and heavy ambitions.

There is perhaps no better way to illuminate the strides taken in conservation today than the SCBI's annual Autumn Conservation Festival. It's the only time each year that the facility is open to the public. Participants visit many booths throughout the facility to learn about current captive as well as wild conservation efforts for many species, from cranes to clouded leopards. Although not the entire facility is available to tour, many of the species residing on the facility are accessible to the public to watch and learn about. Being that I am doing steroid hormone research for clouded leopards, I naturally worked at the clouded leopard booth all weekend. We had sweet temporary tattoos, a fun game for adults and children alike testing their knowledge of wild cat species across the world, and featured MS and PhD projects currently working for clouded leopard conservation.


One of the tables at the clouded leopard booth featured this real leopard pelt. This individual cat was a zoo animal who died of natural causes and now serves as an educational tool. Visitors could touch the fur and see up close the curious, cloud-like pattern that gave the animal it's name. In addition, we discussed how clouded leopards are replacing tigers in the black markets for both fur sales and traditional medicines. Because tiger populations are in decline, hunters and poachers are turning to this smaller cat for their fur, to sell, and body parts (teeth, bones, fluids, meat) for traditional medicine (which, I'm going to say, probably won't cure your headache or impotency problem). Although laws against this exist, they are not strictly enforced in the clouded leopards home range, which extends through Thailand, Vietnam, Laos, Cambodia, and farther south. These cats are arboreal, meaning they live in trees, though researchers do not know how often they spend their time between treetops and the ground. With poachers having better luck at finding these cats than conservationists, clouded leopard populations are unknown as well as unstable. Clouded leopards prey on monkeys, birds, small mammals, and deer. Of all the cats in the world, the clouded leopard has the largest canines relative to the size of it's skull... making it the living relative of the extinct saber-toothed cat. A master's student is currently studying these leopards through the use of non-invasive infrared camera trapping. These cameras, which are soundless as well as flashless, catch photos of wildlife in order to better assess what these cats are doing in their free time. Apparently one species of forest chicken is particularly fond of these cameras, as the student has collected more than one thousand shots of these birds sitting in front of the cameras (which are secured to trees at eye-level for the cats) and turning their heads in the idiotic fashion typical of your average farm chicken (I'm sure the clouded leopards eat them, too).





Meagan talks about clouded leopard physiology




With two booths, we were pretty busy! Heather (pictured right) is a PhD candidate studying male clouded leopard testosterone and cortisol to reduce male aggression towards females in captivity.



Listening intently to one of many very good questions! One of the infrared cameras I mentioned is sitting on top of the pelt. One interesting question I had to answer a lot from the small scientists: "How did you kill the clouded leopard?" WITH A LIGHT SABER! Just kidding.

I managed to score some pretty cool temporary tattoos when I walked around to the different booths! A video of the two female lions at the national zoo meeting the new male for the first time echoed throughout the facility (and my personal interpretation of this interaction consists of things I'm not willing to type out. But, that's just a guess.), drawing people in to learn about current projects to help with their survival. Other fun things included being able to see the maned wolves, checking out how tall some of the crane species are, and seeing some of both the clouded leopards and red pandas up close. I really enjoyed walking around the booths and learning about different conservation efforts, and I also learned a lot! Some highlights included visiting the Global Tiger Initiative booth (congratulations Ryan on the new job!), being able to basically lay down inside the girth of an elephant radio collar (not like the bobcat collars at all!), and consuming a vast amount of homemade potato chips. It was a joy to see the interest the community has in learning about the many species needing our help, and everyone enjoyed discussing what they are most passionate about. All in all, a great weekend!


Red pandas. Are. So. Cute.



 Sa Ming is one of the clouded leopards born at the national zoo. Here, he is seen wreaking general havoc.


A good still shot 



Yea!



One of the girls- I have not learned her name- having a good sniff.

Front Royal, Virginia

I have just arrived in Front Royal, VA to begin an internship with the Smithsonian Conservation Biology Institute. I am so excited! I start tomorrow on a project investigating fecal hormones for the purpose of in vitro fertilization (IVF) in the clouded leopard (Neofelis nebulosa). Using domestic cats as a model species, the project aims to optimize oocyte quality for IVF in the clouded leoard, as clouded leopards have difficulty breeding in captivity. Male aggression towards females in natural mating can often be fatal for the females, with the chance of losing limbs also high. With other projects studying ways to mitigate male aggression, artificial insemination of clouded leopards has also been examined. Clouded leopards are spontaneous ovulators, and combined with lack of response to hormone stimulation, artificial insemination has proved ineffective for these cats. I will be working for postdoc Dr. Rebecca Hobbs assessing how domestic cats respond to hormones such as estrogen and progestin for the purpose of developing a successful program of IVF for the extremely endangered clouded leopard.

I am staying just a few steps from the laboratory I will be working in. The dorm houses many interns during different semesters, and there are currently seven or eight women this fall. I've met three of my housemates in addition to a visiting cheetah scientist, and the backgrounds and scientific interests I've come across are amazing! My neighbors other than the interns include bison, cranes (cannot remember what species... will find out), and maned wolves. The cranes make a bit of noise at night and they sound like the combination between a loon and large woodpecker hacking away at a tree. Come breeding season in November, I have been promised sleepless nights. The bison are... big. I can see their field from my bedroom window, and the maned wolves are located throughout the compound and call to each other in the night (I am waiting to hear this). Since I've only seen the back end of one of these very red, very tall wolves, I cannot yet remark on their tall legs and glorious manes. I can, however, say that they are very sneaky. Other animals here include Przewalski's horses; onagers (a Persian donkey); Eld's deer; red pandas; cheetahs; and clouded leopards.

I arrived yesterday after driving from Whitefish, MT, to Houston, TX. The job offer came three weeks ago, so the whole process has been rather fast. I miss Montana and Bobbie already! As of when I left, we had yet to catch either of the two bobcats with the failed collars. This week, however, Bobbie has managed to catch a few more bears in her traps, so the excitement in Flathead National Forest never ends.

Training starts tomorrow- wish me luck!

Interesting Video

I dreamt last night I was at a wildlife sanctuary that housed rescued lions. In the dream, a small group of people entered the enclosure containing one lion and two lionesses. I was one of the people in the group. The entire dream consisted of this small group trying to not get bitten by these lions. Unfortunately, the male lion kept coming up to me and rubbing his heavy mane along my midsection, repeatedly mouthing my arms and legs in the way that small puppies do when they are teething. I spent the entire dream terrified that this lion was going to do what he was made to do: eat meat. Photographers were nearby trying to capture these moments in "conservation." While I often wake up wondering what the heck is going on in my subconscious, this dream was such a healthy reminder that although I love cats, I would be scared out of my mind to force shared space with one. That's just not smart! When I came across the following over coffee this morning, I couldn't have found a better example of why captive animals need respect, too!

I first saw this video courtesy of Big Cat Rescue, a wildlife sanctuary in Tampa, Florida. While I recognize that the benefit was for tigers and some sort of conservation, the message sent through this video does not positively support tiger conservation or accurate tiger behavior. I want to share it because there are so many things going wrong!  Foremost, it is not a tiger attack. This tiger, who was already clearly overstimulated and showing signs of stress, is very excited about the cologne the man is wearing and is trying to bask in the scent. Second, why did said man see fit to wrap his arms around a tiger's neck? Thirdly (that is actually a word!), the tiger should not have had the opportunity to get close to anyone. And finally, what the hell was a tiger doing at a fundraiser? Even if you don't know tiger behavior, it is easy to imagine how very quickly the situation could have gone awry and that man would have been killed. Such irresponsible tiger promotion! Both parties are very lucky, as the animal probably would have been destroyed for mauling a human, and well, the guy's luck is pretty self-explanatory.


Tiger Attacks At Fundraiser!

Trapping Bobcats

In light of the new and exciting information that two of our bobcats were not dead, we have instead had to deal with two collars failing instead of bobcats... meaning that we have to trap two bobcats in 250,000 acres of national forest in order to remove and replace said collars. Where is an easy button when you need it?! Luckily (relatively speaking, that is), we've been able to follow the bobcats via telemetry to get an idea of their whereabouts. After following M6 around for three days on foot, the obvious decision from that point onward was to only set a trap when we determined what area the cat was hanging out in. The collars send a retrieval signal (which sounds dangerously like a mortality signal... thanks fine print manual) 24/7 once the collar battery fails, so really we only have a small window of time to collect the collar. If the battery dies completely, no more signal and our cat is as unknown as it was before being collared.

For the past few weeks we've been setting and checking traps. Three of the six traps set were collapsible, similar to the have-a-heart design and non-fatal. The other three were much larger, weighing almost forty pounds and hand-built by Bobbie. The traps are double-wired, and this past winter one of her bobcats chewed through the wire. These traps take quite a beating, as the cats basically ping-pong about inside the trap once the gate slams shut. In winter, when the bears were hibernating, Bobbie used meat as a lure. One bobcat, and the largest of the collared cats, was caught twelve times this past winter because he learned that Bobbie wasn't going to hurt him and that he could get a free meal each time he went into the trap. In one case, this cat ate almost fifteen pounds of deer meat in a night! The cat weighs thirty five pounds (which is rather large for a bobcat)! That would be like me eating fifty pounds of meat... okay, I'm totally lying about my weight, but that's a lot of food. Now that it's summer, the bears are out and about, and meat in a trap is no longer an option. Bears are more opportunistically carnivorous, but they do enjoy a bit (or a lot) of meat now and then. Since we don't have any bear-proof traps, using meat would mean certain death for the traps, a danger to us, and no bobcat in our traps. We decided to trap M5 first, and instead of meat used the always delightful scent lures. These little glass bottles contain all that is disgusting to the human nose, and all of it is au naturale. Be it skunk musk, bobcat scent glands, salmon oil, or the mysterious lure No. 2 (very popular amongst cats), a drop rubbed on the base of a tree- bobcat height- will attract all sorts of wildlife just dying to get a better whiff. Once we set traps, I traipsed about animal trails making a scent trail for our nearby bobcat to follow. Curiosity always killed the cat, so the scent lures are intended to lead the animal within site of the trap. We also hung blank CD's from tree branches to attract the curious cats ("oooooh, shinyyyy"), and we used salmon oil only inside the trap to provide incentive to enter the trap. Should the animal apply pressure with his paw, the door slams and the cat is secure until we arrive. The traps were covered with branches to shield the animal from weather or human eye, and we began to check the traps every day.

Checking for traps is tedious and always a waiting game. The animal may move from the area, be too wary of the trap, or wait several days before considering approaching the foreign object. Or, if he's been trapped before, like ours, he could be trap shy. Since it's summer, we've had to be extremely careful of bears being in or around the traps when we approach them. I was nervous on the first day checking traps! I felt a little like Chuck Norris with my hand on the trigger of my bear spray as we climbed into the trees toward traps. All of my bear experiences thus far have been from either the truck or with lots of trees between us with me yelling "bad bear!" Day after day, however, the traps have been empty. We were extra careful in setting the traps: we wired them to trees so nothing could carry them off; we hid them from roads but in areas open enough that we might see anything dangerous as we approached; and most importantly, we did not get any of the scent lures on our hands! Still, wildlife is wild and nothing is predictable.

The day we set our fourth trap, there was either a moose or a bear nearby in the woods interested in our work, though lots of yelling rendered the stranger silent. Walking down animal trails can be quite unnerving since everything uses them. I have substantially freaked myself out while setting traps because I've walked quite a bit away from Bobbie to mark trails, where strangers lurk. Thinking like a bobcat requires a lower sense of balance, and being low to the ground and having the hairs on the back of your neck stand up is truly a wild feeling! Twice I very seriously felt like something was watching me, and not a hare or a squirrel. Could have been a mountain lion, a baby moose, or a bear. This day, and one other, I'm comfortable not knowing. After returning from the trail and humming quite loudly to myself, I suddenly came face to face with a grouse. The silly little bird had somehow gotten between Bobbie and I undetected and was stupidly walking about like the chickens I grew up laughing at. To make a very long story short, we ended up chasing that grouse (what bear wants to eat a grouse?), and I am so happy that no one was there to film it, because the grouse won 2:1. We did, however, use some donated feathers for our trap, and confirmed with the squirrels that we are insane with our hysterical laughter and tripping over trees. This very trap was to hold some excitement in the coming days.

I am so angry that I was not actually here for this. This past Saturday was the first and only day all season I have missed. I woke with a terrible migraine and it rendered me totally useless. Traps have to be checked, so Bobbie ventured out this past weekend to check traps. When she got to the trap with the grouse feathers, she noticed the trap door was closed. Checking for M5's signal and not finding it, she assumed there was another bobcat in the trap, as all was silent. The trap was well covered so she could see nothing as she approached the trap. Bobbie was ten feet from the trap when it exploded into the air, leaping from the ground as if alive and being thrown over the saplings. Inside the larger trap burst a very frantic black bear, blasting at lightning speed past Bobbie and off into the trees, hollering the whole time like a cub though it was a young adult. Bobbie says she basically levitated back to the truck, only emerging to check out the trap after she had relearned how to breath from inside the vehicle. When we both returned the next day to collect the trap, I could see where the bear had broken through both sets of wire as well as snapping the heavy pvc like a twig. Good thing he was the more frightened of the two! We are fairly certain it was the yearling cub we saw at the beginning of July (the one standing up in the picture in a previous posting). Either way, it most certainly was not a bobcat, and I most certainly am still disgruntled that I missed the excitement!

To date, we have still not trapped either of the sneaksy, tricksy little cats. However, the Montana field season has been nothing short of exhilarating!

Bobcat Collars: FAIL

After finishing a study site just moments before the sky began to spit out teardrops, Bobbie and I happily climbed into the truck to head down the mountain. Just as we crossed onto the paved part of the road (which means home is close because one can drive fast!), we changed one of the channels on the receiver to see if one of the bobcats was in the area. Hearing only static, we were about to turn off the receiver (the signal comes via GPS through the collars on each cat. An antenna on the collar allows us to know if we are within two miles of an animal. We have a hand held transmitter, and also one glorious magnetic antenna that sits on top of the truck and allows us to cover ground more quickly), we heard the double clicking signal. The signal emits a heartbeat-like beeping on a live animal, but a rapid clicking serves as a mortality signal. My heart sank as I heard my first mortality signal, and we began a triangulation to determine the relative location of the cat. A triangulation is putting three compass bearings and their respective UTM's into the GIS for a fairly accurate location for an animal. We take the hand-held antenna and point it in the direction that the signal is loudest, narrowing it down to a directional line. That line is used for a compass bearing, and we then travel ninety degrees in a circle (hopefully!) to determine yet another bearing. It can take six or seven times to get enough points at varying angles so the GIS will determine a location (via computer). That being said, Bobbie and I didn't have a computer, so we tried to determine a relative location for our cat. We then grabbed our gear and headed out to find the body. Unsure of what we would find, we had our bear spray in case our cat had been killed by a bear or mountain lion. The telemetry led us into a mature forest with lots of fallen logs... sadly a place that a bobcat would live, and also choose to die. We commenced our search, but we very quickly ran into a problem: the signal was everywhere. Telemetry is very simple: the closer you get to your target, the louder the signal. Ideally, an unmoving object presents a fairly easy target. However, our deceased animal's signal was not only getting louder, but it was louder a hundred yards across the trees. We searched in fallen branches, in stumps (I did work for almost ten minutes hacking into a cedar log. You actually cannot hack into a cedar log.), and up in trees for any sign of a carcass or collar. The pieces might not have been together, so we searched for anything. As it started to rain, I heard my first elk... and it is a strange sound. Way up on a hill, we could hear a startled and irate bull elk snorting at us repeatedly. After adjusting to the sound, we continued to curse and scratch our heads over the strange signal. We found traces of what we thought was bobcat and deer hair, but nothing else. After an hour, we went home soaking wet, empty handed, and disappointed.

The rest of the week revolved around finding this dead bobcat. We determined that the signal must be bouncing off of the many ridges of the mountains in this one area as well as the river running through the valley. Bobbie and I spent the next two days hiking for the cat with three paws. This bobcat was caught in a furbearer trap this past December (a conibear trap), but escaped only because he chewed his own paw off. I digress to marvel at the fierce instinct of survival this cat had to chew off his own paw. Our search for this amazing cat, whose fate was still indeterminate, led us to a cliff over the river:


The signal was leading us down into the valley. The river running through the valley was swift, with picturesque rapids and contrasting clear, still moments on the rocks. From the cliff we leaned over we could see how deep the river was in some parts, and there was one rock in the center of the riverbed that was easily four times the size of my car, and much taller, that was covered completely by the crystal clear water. The path down, however, was very down, and the slope was covered by rocks that had fallen off the cliff in an avalanche. Making our way down, we determined that not only was the signal inconsistent, but it was leading us across the river.

Crawling down to the bank, we looked at the water, then at each other, and Bobbie said, "I'm game if you are." Pumping myself up to cross this river was completely crazy! We debated leaving on our boots, but we found a more shallow area to cross (only thigh high) and decided to take off our boots. I know I definitely did not notice how cold the water was, as I was intent on not losing my balance over the sharp and slippery rocks that were urging the water to rip me off my feet. Don't get me wrong- we weren't going to die, probably- but I don't know anyone who is relaxed about getting swept down a fifty degree river with expensive equipment in their pack.


Bobbie snapped this picture on the way through the river the second time. She snapped the pic as I was lifting my right leg to take another shuffle, but I almost lost my balance. The other, and more dignified photo, shows me with both arms going into the water and trying to straddle the rocks with four limbs and keep my footing.


The journey in between these photos gave us new insight to the term "going in circles," because that is exactly what we did! The signal got loud, then faint, then took us left, then up, then right and down. We found wild strawberries, moose bones, and I heard my first osprey. We had just decided to give up, and I had long ago determined that I had no idea where we were in relation to the river, when we rounded some trees and came upon the giant rock we ate lunch on. We had come full circle! This is not good, because the whole goal of the day was to find our deceased bobcat.

Getting back to the truck, we discussed our options, concerned we would have to fly a helicopter to find this cat (no joke). The next day, however, the signal was in a different location entirely. From the road, the signal was coming and going. That usually only happens when a bobcat is active. Active = Alive. Hmmmmm. This cat, M6, was one of two cats wearing a different brand of radio collar. The next day we decided to search for the other collared bobcats to see what their signals sounded like. Driving the roads, we found each cat, and each signal was a live signal, except for M5. He, too, had the same mortality signal, and he is the other cat with the other brand of radio collar. The collars have a live signal, a mortality signal, and a recovery signal when the battery is dying. Nature is unpredictable, yet the coincidence of having two cats dead within 48 hours, combined with the inconsistency of the signal strength, pointed to one very enlightening fact: our cats were alive! Poor M6 had been moving in the woods for two days trying to get away from the crazy beeping ladies, and we had been tailing him the entire time! After our excursions, we managed to discover that the mortality signal and the recovery signal on the transmitter is only slightly different (why this was highlighted in a very tiny footnote instead of a bold headline in the manual is beyond me).

Long story short, no cat was harmed in the making of this adventure. The company who makes the collars that failed after only four months is still intact, and we are currently wrapping up the field season by trying to catch these bobcats so we can retrieve the information on the collars!